How the reverse supply chain contributes to a firm’s competitive strategy: a strategic alignment perspective

The ongoing transition to a circular economy is changing the shape of supply chains. They are becoming more ‘Closed-Loop’, combining forward and reverse flows of products and materials. Reverse supply chains (RSCs), originally considered as a solution for handling waste or recovering residual value, can play a pivotal role in determining the competitive advantage of the firm. Firms do not always exploit the potential of the RSC, and the conditions allowing the exploitation remain unclear. This paper explores the alignment between the RSC and the competitive strategy of the firm. Results from seven case studies, focusing on original equipment manufacturers, show how the RSC can play a strategic, tactical or operational role for the firm. The paper applies for the first time the concept of strategic alignment to the RSC, and practitioners can use the proposed framework to analyse the role of the RSC within their firm

Permafrost meta-omics and climate change

Permanently frozen soil, or permafrost, covers a large portion of the Earth's terrestrial surface and represents a unique environment for cold-adapted microorganisms. As permafrost thaws, previously protected organic matter becomes available for microbial degradation. Microbes that decompose soil carbon produce carbon dioxide and other greenhouse gases, contributing substantially to climate change. Next-generation sequencing and other -omics technologies offer opportunities to discover the mechanisms by which microbial communities regulate the loss of carbon and the emission of greenhouse gases from thawing permafrost regions. Analysis of nucleic acids and proteins taken directly from permafrost-associated soils has provided new insights into microbial communities and their functions in Arctic environments that are increasingly impacted by climate change. In this article we review current information from various molecular -omics studies on permafrost microbial ecology and explore the relevance of these insights to our current understanding of the dynamics of permafrost loss due to climate change

’’I Did It My Way’’? – en sociologisk samtidsdiagnose over den senmoderne/postmoderne død

I denne artikel beskrives de senere års forandringer i dele af den danske døds- og mindekultur med fokus på, hvorledes den enten adskiller sig fra eller mere skal ses som en forlængelse af den i samfundsforskningen ofte fremhævede, fortrængte og tabuiserede dødskultur fra det 20. århundrede. Gennem artiklen diskuterer og illustrerer forfatterne mange af nutidens forandringer gennem en række nedslag i form af et fokus på omsorgen for den døende, individualiseringen af institutionelle og professionelle praksisser, personliggørelsen af ritualer og ceremonier, udfordringen af traditioner og konventioner og den ændrede betydning af kirkegården og mindekulturen. Formålet med artiklen er således at beskrive og dokumentere nogle af de mest centrale ændringer i vores samfunds dødskultur som et spejlbillede på mange af de øvrige samfundsforandringer, der udspiller sig. Derudover ønsker forfatterne at anspore til debat om, hvorvidt den udbredte postmoderniseringstese om valgfrihed, fleksibilitet og individualisering inden for dødsområdet kan underbygges, eller om den skal suppleres med en senmoderniseringstese, der har fokus på institutionel kontrol og inerti. Søgeord: Senmoderne død, postmoderne død, institutioner, ritualer, mindekultur, professionalisering. ENGELSK ABSTRACT: Janet Ferrari Wanseele and Michael Hviid Jacobsen: ”I did It My Way”? – A Sociological Contemporary Diagnosis of Late Modern/Post Modern Death This article describes some of the major changes taking place in the contemporary culture of death, dying and bereavement in Denmark. It does so by analysing how these may be contrasted with or seen as a continuation of the often advanced thesis of a death-denying and death-taboo culture of the 20th century. The authors illustrate many of the changes through analysis of selected topics such as the care of the dying, the individualization of institutional and professional practice, the personalized content of rituals and ceremonies, the challenge to traditions and conventions and the changing mentality regarding resting place and memorial culture. The purpose of the article is to describe and document some of the major shifts in death and dying as a corollary to contemporary changes within the culture at large. In addition the authors speculate as to whether the current popularity of the thesis of a postmodernization of death and dying can be substantiated or whether it needs to be supplemented by a late modern thesis concerned with institutional control and inertia. Key words: Late modern death, postmodern death, institutions, rituals, memorial culture, professionalization

The Catalytic Site Atlas 2.0: cataloging catalytic sites and residues identified in enzymes.

Understanding which are the catalytic residues in an enzyme and what function they perform is crucial to many biology studies, particularly those leading to new therapeutics and enzyme design. The original version of the Catalytic Site Atlas (CSA) (http://www.ebi.ac.uk/thornton-srv/databases/CSA) published in 2004, which catalogs the residues involved in enzyme catalysis in experimentally determined protein structures, had only 177 curated entries and employed a simplistic approach to expanding these annotations to homologous enzyme structures. Here we present a new version of the CSA (CSA 2.0), which greatly expands the number of both curated (968) and automatically annotated catalytic sites in enzyme structures, utilizing a new method for annotation transfer. The curated entries are used, along with the variation in residue type from the sequence comparison, to generate 3D templates of the catalytic sites, which in turn can be used to find catalytic sites in new structures. To ease the transfer of CSA annotations to other resources a new ontology has been developed: the Enzyme Mechanism Ontology, which has permitted the transfer of annotations to Mechanism, Annotation and Classification in Enzymes (MACiE) and UniProt Knowledge Base (UniProtKB) resources. The CSA database schema has been re-designed and both the CSA data and search capabilities are presented in a new modern web interface

Mechanisms underlying the exquisite sensitivity of Candida albicans to combinatorial cationic and oxidative stress that enhances the potent fungicidal activity of phagocytes

Copyright © 2014 Kaloriti et al.Peer reviewedPublisher PD

Integrative Model of Oxidative Stress Adaptation in the Fungal Pathogen Candida albicans

Acknowledgments We are grateful to the Ian Fraser Cytometry Centre and our Mass Spetrometry and qPCR Facilities for help with the flow cytometry, glutathione and qRT-PCR assays, respectively. We also thank our many colleagues in the CRISP Consortium and in the medical mycology and systems biology communities for insightful discussions. Funding: This work was supported by the CRISP project (Combinatorial Responses In Stress Pathways), which was funded by the UK Biotechnology and Biological Research Council (www.bbsrc.ac.uk): AJPB, KH, CG, ADM, NARG, MT, MCR. (Research Grants; BB/F00513X/1, BB/F005210/1-2). AJPB and JQ received additional support from the BBSRC (Research Grants; BB/K016393/1; BB/K017365/1). NARG and AJPB were also supported by the Wellcome Trust (www.wellcome.ac.uk), (Grants: 080088; 097377). AJPB was also supported by the European Research Council (http://erc.europa.eu/), (STRIFE Advanced Grant; ERC-2009-AdG-249793). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Snapshot of iron response in Shewanella oneidensis by gene network reconstruction

<p>Abstract</p> <p>Background</p> <p>Iron homeostasis of <it>Shewanella oneidensis</it>, a γ-proteobacterium possessing high iron content, is regulated by a global transcription factor Fur. However, knowledge is incomplete about other biological pathways that respond to changes in iron concentration, as well as details of the responses. In this work, we integrate physiological, transcriptomics and genetic approaches to delineate the iron response of <it>S. oneidensis</it>.</p> <p>Results</p> <p>We show that the iron response in <it>S. oneidensis </it>is a rapid process. Temporal gene expression profiles were examined for iron depletion and repletion, and a gene co-expression network was reconstructed. Modules of iron acquisition systems, anaerobic energy metabolism and protein degradation were the most noteworthy in the gene network. Bioinformatics analyses suggested that genes in each of the modules might be regulated by DNA-binding proteins Fur, CRP and RpoH, respectively. Closer inspection of these modules revealed a transcriptional regulator (SO2426) involved in iron acquisition and ten transcriptional factors involved in anaerobic energy metabolism. Selected genes in the network were analyzed by genetic studies. Disruption of genes encoding a putative alcaligin biosynthesis protein (SO3032) and a gene previously implicated in protein degradation (SO2017) led to severe growth deficiency under iron depletion conditions. Disruption of a novel transcriptional factor (SO1415) caused deficiency in both anaerobic iron reduction and growth with thiosulfate or TMAO as an electronic acceptor, suggesting that SO1415 is required for specific branches of anaerobic energy metabolism pathways.</p> <p>Conclusion</p> <p>Using a reconstructed gene network, we identified major biological pathways that were differentially expressed during iron depletion and repletion. Genetic studies not only demonstrated the importance of iron acquisition and protein degradation for iron depletion, but also characterized a novel transcriptional factor (SO1415) with a role in anaerobic energy metabolism.</p

Evidence for an FU Orionis-like Outburst from a Classical T Tauri Star

We present pre- and post-outburst observations of the new FU Orionis-like young stellar object PTF 10qpf (also known as LkHa 188-G4 and HBC 722). Prior to this outburst, LkHa 188-G4 was classified as a classical T Tauri star on the basis of its optical emission-line spectrum superposed on a K8-type photosphere, and its photometric variability. The mid-infrared spectral index of LkHa 188-G4 indicates a Class II-type object. LkHa 188-G4 exhibited a steady rise by ~1 mag over ~11 months starting in Aug. 2009, before a subsequent more abrupt rise of > 3 mag on a time scale of ~2 months. Observations taken during the eruption exhibit the defining characteristics of FU Orionis variables: (i) an increase in brightness by > 4 mag, (ii) a bright optical/near-infrared reflection nebula appeared, (iii) optical spectra are consistent with a G supergiant and dominated by absorption lines, the only exception being Halpha which is characterized by a P Cygni profile, (iv) near-infrared spectra resemble those of late K--M giants/supergiants with enhanced absorption seen in the molecular bands of CO and H_2O, and (v) outflow signatures in H and He are seen in the form of blueshifted absorption profiles. LkHa 188-G4 is the first member of the FU Orionis-like class with a well-sampled optical to mid-infrared spectral energy distribution in the pre-outburst phase. The association of the PTF 10qpf outburst with the previously identified classical T Tauri star LkHa 188-G4 (HBC 722) provides strong evidence that FU Orionis-like eruptions represent periods of enhanced disk accretion and outflow, likely triggered by instabilities in the disk. The early identification of PTF 10qpf as an FU Orionis-like variable will enable detailed photometric and spectroscopic observations during its post-outburst evolution for comparison with other known outbursting objects.Comment: 14 pages, 11 figures, ApJ accepte